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Experimental factors and evaluated levels for the FFD and CCD of the extraction
| Experimental factors | FFD | CCD | ||||||
|---|---|---|---|---|---|---|---|---|
| − | 0 | + | − | − | 0 | + | ++ | |
| Temperature (°C) | 7 | 22 | 37 | 0 | 2 | 7 | 12 | 14 |
| pH | 6.5 | 7.5 | 8.5 | |||||
| Time (h) | 3 | 6 | 9 | |||||
| AmBic (mM) | 25 | 50 | 75 | |||||
| DTT (mM) | 25 | 50 | 75 | |||||
| Urea (M) | 3 | 6 | 9 | 2.8 | 3.7 | 5.8 | 7.9 | 8.7 |
