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Experimental factors and evaluated levels for the FFD and CCD of the digestion
| Experimental factors | FFD | CCD | ||||||
|---|---|---|---|---|---|---|---|---|
| − | 0 | + | − | − | 0 | + | ++ | |
| Time (h) | 2 | 5 | 8 | 9 | 11.25 | 13.5 | 15.75 | 18 |
| AmBic (mM) | 25 | 50 | 75 | 0 | 6.25 | 12.5 | 18.75 | 25 |
| DTT (mM) | 25 | 50 | 75 | 0 | 6.25 | 12.5 | 18.75 | 25 |
| ACN (%*) | 0 | 5 | 10 | |||||
| DMSO (%*) | 0 | 5 | 10 | 0 | 6.25 | 12.5 | 18.75 | 25 |
*Final volume
