Table 3.
Overall relative variance for the repeat analysis of milk- and egg-derived peptides. Contributions expressed as % relative from extraction, digestion and LCMS peak area ratio measurements
| Protein | Peptide | Source of variability | SS | MS | Total CV% | Variance % of total |
|---|---|---|---|---|---|---|
| αS1 | YLGYLEQLLR* | Extraction | 0.146 | 0.073 | 5 | 14.0 |
| Digestion | 0.280 | 0.047 | 67.8 | |||
| Ratio measurement | 0.069 | 0.004 | 18.2 | |||
| FFVAPFPEVFGK | Extraction | 2.29666E+12 | 1.14833E+12 | 10.3 | 0.8 | |
| Digestion | 6.6312E+12 | 1.1052E+12 | 41.5 | |||
| Ratio measurement | 6.30122E+12 | 3.50068E+11 | 57.7 | |||
| αS2 | VIPYVR | Extraction | 65,911,263,315 | 32,955,631,658 | 6.4 | 2.0 |
| Digestion | 1.22099E+11 | 20,349,773,838 | 0.0 | |||
| Ratio measurement | 9.14193E+11 | 1.01577E+11 | 98.0 | |||
| FALPQYLK* | Extraction | 0.546 | 0.273 | 5.6 | 0.0 | |
| Digestion | 1.694 | 0.282 | 75.4 | |||
| Ratio measurement | 0.356 | 0.040 | 24.6 | |||
| NAVPITPTLNR | Extraction | 6.12179E+11 | 3.0609E+11 | 45 | 0.4 | |
| Digestion | 9.24282E+11 | 1.54047E+11 | 0.0 | |||
| Ratio measurement | 6.32202E+13 | 7.02446E+12 | 99.6 | |||
| ALNEINQFYQK | Extraction | 21,969,405,628 | 10,984,702,814 | 10.9 | 0.0 | |
| Digestion | 88,508,367,129 | 14,751,394,522 | 42.0 | |||
| Ratio measurement | 83,726,462,791 | 4,651,470,155 | 58.0 | |||
| βCN | VLPVPQK | Extraction | 3.62137E+13 | 1.81068E+13 | 40 | 17.7 |
| Digestion | 6.57953E+13 | 1.09659E+13 | 81.4 | |||
| Ratio measurement | 6.92243E+11 | 38,457,965,145 | 0.9 | |||
| AVPYPQR | Extraction | 6.2232E+12 | 3.1116E+12 | 37.2 | 0.0 | |
| Digestion | 2.13986E+13 | 3.56643E+12 | 92.8 | |||
| Ratio measurement | 1.61913E+12 | 89,951,794,843 | 7.2 | |||
| κCN | YIPIQYVLSR | Extraction | 1.14481E+12 | 5.72405E+11 | 6.4 | 2.8 |
| Digestion | 2.99726E+12 | 4.99543E+11 | 38.5 | |||
| Ratio measurement | 3.03071E+12 | 1.68373E+11 | 58.7 | |||
| βLG | GLDIQK | Extraction | 9,374,383,991 | 4,687,191,996 | 23.1 | 0.0 |
| Digestion | 29,866,046,898 | 4,977,674,483 | 79.0 | |||
| Ratio measurement | 5,269,055,283 | 585,450,587.1 | 21.0 | |||
| IPAVFK | Extraction | 76,749,448,703 | 38,374,724,352 | 8.7 | 4.5 | |
| Digestion | 1.81446E+11 | 30,241,083,290 | 27.4 | |||
| Ratio measurement | 2.46673E+11 | 13,704,048,151 | 68.1 | |||
| ALPMHIR | Extraction | 4,533,019,326 | 2,266,509,663 | 9.6 | 0.0 | |
| Digestion | 34,287,299,411 | 5,714,549,902 | 2.0 | |||
| Ratio measurement | 97,025,982,779 | 5,390,332,377 | 98.0 | |||
| Ova | LYAEER* | Extraction | 0.039886019 | 0.019943009 | 7.3 | 7.0 |
| Digestion | 0.091391929 | 0.015231988 | 55.0 | |||
| Ratio measurement | 0.051301231 | 0.002850068 | 38.0 | |||
| GLWEK | Extraction | 3.13527E+11 | 1.56763E+11 | 11.8 | 0.1 | |
| Digestion | 9.37364E+11 | 1.56227E+11 | 70.8 | |||
| Ratio measurement | 3.38734E+11 | 18,818,572,806 | 29.1 | |||
| HIATNAVLFFGR | Extraction | 4.15205E+11 | 2.07602E+11 | 8.9 | 36.7 | |
| Digestion | 2.9878E+11 | 49,796,694,363 | 20.4 | |||
| Ratio measurement | 3.68934E+11 | 20,496,340,998 | 42.9 | |||
| Lys | GTDVQAWIR | Extraction | 53,321,662,879 | 26,660,831,440 | 7.4 | 33.6 |
| Digestion | 49,068,398,641 | 8,178,066,440 | 33.8 | |||
| Ratio measurement | 35,821,898,572 | 1,990,105,476 | 32.6 | |||
| FESNFNTQATNR* | Extraction | 0.006200414 | 0.003100207 | 6.5 | 0.0 | |
| Digestion | 0.022979051 | 0.003829842 | 72.5 | |||
| Ratio measurement | 0.007724225 | 0.000429124 | 27.5 | |||
| HGLDNYR | Extraction | 39,193,790.63 | 19,596,895.32 | 28.4 | 0.0 | |
| Digestion | 856,334,672.1 | 142,722,445.3 | 86.4 | |||
| Ratio measurement | 127,696,469.5 | 7,094,248.306 | 13.6 | |||
| Myo | Extraction | 4.88984E+12 | 2.44492E+12 | 5.3 | 24.8 | |
| Digestion | 4.70281E+12 | 7.83802E+11 | 15.1 | |||
| Ratio measurement | 8.04677E+12 | 4.47043E+11 | 60.1 | |||
| Glu-Fib | Extraction | 1.2567E+12 | 6.28352E+11 | 14 | 9.4 | |
| Digestion | 1.43487E+12 | 2.39144E+11 | 0.0 | |||
| Ratio measurement | 3.42278E+12 | 2.0134E+11 | 90.6 |
*Peptides for which an isotope-labelled peptide standard was available