KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse Anti-Human CD34 clone 581 | BD Biosciences | Cat#560710; RRID: AB_2687922 |
| Mouse Anti-Human CD34 clone 8G12 | BD Biosciences | Cat#348057; RRID: AB_2687922 |
| Rat Anti-Human CD49f clone GoH3 | BD Biosciences | Cat#555736; RRID: AB_396079 |
| Rat anti-mouse CD45 clone 30-F11 | Bio Legend | Cat#103126; RRID: AB_493536 |
| Mouse Anti-Human CD45 clone HI30 | BD Biosciences | Cat#560777; RRID: AB_1937324 |
| Mouse Anti-Human CD143 (ACE) clone BB9 | BD Biosciences | Cat#557813; RRID: AB_396883 |
| Mouse Anti-Human CD90 clone 5E10 | eBioscience | Cat#17–0909-42; RRID: AB_953611 |
| Mouse Anti-Human CD38 clone HIT2 | BD Biosciences | Cat#560676; RRID: AB_1727472 |
| Biotin Anti-Human CD133 clone AC133 | Miltenyi Biotec | Cat#130–090-664; RRID: AB_244341 |
| Mouse Anti-Human CD3 clone UCHT1 | eBioscience | Cat#47–0038-42; RRID: AB_906221 |
| Mouse Anti-Human CD19 clone HIB19 | Biologend | Cat#302254; RRID: AB_2564142 |
| Mouse Anti-Human CD11c clone 3.9 | Biologend | Cat#301618; RRID: AB_439791 |
| Mouse Anti-Human CD14 clone HDC14 | Biologend | Cat#325618; RRID: AB_830691 |
| Diamond human CD34 isolation kit | Miltenyi Biotec | Cat#130–094-531; RRID: AB_2721154 |
| Mouse Monoclonal Anti-FLAG clone M2 | Sigma Aldrich | Cat#F1804; RRID: AB_259529 |
| Rabbit polyclonal Anti-HA clone 4C12 | Abcam | Cat#ab9110; RRID: AB_10637297 |
| Rabbit polyclonal Anti-FOS clone 4 | Santa Cruz | Cat#sc-52; RRID: AB_2106783 |
| Normal rabbit IgG | Santa Cruz | Cat#sc-2025; RRID: AB_737196 |
| Mouse monoclonal β-actin clone AC-74 | Sigma Aldrich | Cat#A2228; RRID: AB_476697 |
| Bacterial and Virus Strains | ||
| Competent E. coli DH5α | NEB | Cat#C2987I |
| Biological Samples | ||
| Cord Blood | New York Blood Center | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Polybrene | Sigma | Cat#H9268 |
| Doxycycline hyclate | Sigma | Cat#D9891 |
| Dulbecco’s Modified Eagle Medium (DMEM) | GIBCO | Cat#11965–092 |
| L-Glutamine | GIBCO | Cat#25030–081 |
| Penicillin-Streptomycin Solution | GIBCO | Cat#15140–122 |
| FBS | BenchMark | Cat#100–106 |
| TryPLE Express | GIBCO | Cat#12605–010 |
| Accutase Cell detachment solution | Innovative Cell Technologies | Cat#AT104 |
| Dulbecco’s Phosphate Buffer Solution (DPBS) | GIBCO | Cat#14190–144 |
| Myelocult Media | Stem Cell Technologies | Cat#5150 |
| Hydrocortisone | Stem Cell Technologies | Cat#07904 |
| Trizol Reagent | Ambion RNA | Cat#15596026 |
| ECL | Thermo Scientific | Cat#32209 |
| Femto | Thermo Scientific | Cat#34095 |
| Protein G Agarose beads | Roche | Cat# 11719416001 |
| Protein G Dynabeads | Life Technologies | Cat#10004D |
| Proteinase K | Invitrogen | Cat#100005393 |
| RNase A | 5Prime | Cat#2900403 |
| Phenol/Chloroform/Isoamyl Alcohol | Fisher | Cat#BP1752 |
| Sodium Acetate | Sigma | Cat#S7899 |
| GlycoBlue | Life Technologies | Cat#AM9515 |
| Glycine | Fisher | Cat#G48–212 |
| Formaldehyde solution | Sigma | Cat#F8775 |
| Gelatin from Porcine Type A | Sigma | Cat#G1890–100 |
| Molecular grade water | Corning | Cat#46–000-1 |
| BSA | Fisher | Cat#BP1600 |
| BES buffered saline solution | Sigma | Cat#14280–100 |
| Protease Inhibitor cocktail tablets | Roche | Cat#1187358001 |
| Critical Commercial Assays | ||
| Nextera XT library preparation kit | Illumina | Cat#FC-121–1031 |
| TruSeq RNA Sample Prep kit | Illumina | Cat#RS-122–2001 |
| KAPA Hyper Prep Kit | KAPA Biosystems | Cat#KK8502 |
| NEBNext ChIP-seq Library Prep Master Mix Set | New England Biolabs | Cat#E6240L |
| Agilent High Sensitivity DNA kit | Agilent Technologies | Cat#5067–4626 |
| Agilent RNA 6000 Nano kit | Agilent Technologies | Cat#5067–1511 |
| Diamond CD34 Isolation kit | Miltenyi Biotec | Cat#130–094-531 |
| C1 Single-Cell Reagent kit | Fluidigm | Cat#100–6201 |
| SMARTer Ultra Low RNA kit for the Fluidigm C1 System | Clontech | Cat#634833 |
| Quant-iT PicoGreen double-stranded DNA Assay kit | Thermo Fisher Scientific | Cat#P7589 |
| AMPure XP beads | Beckman Coulter | Cat#A63881 |
| Deposited Data | ||
| Bulk RNA-seq, Single Cell RNA-seq, ChIP-seq data | This paper | Gene Expression Omnibus (GEO): GSE51025 |
| Experimental Models: Cell Lines | ||
| Human adult dermal fibroblasts (HDF) | ScienCell | Cat#3220 |
| Neonatal foreskin fibroblasts (BJ) | ATCC | Cat#CRL-2522 |
| HEK293T | ATCC | Cat#CRL-3216 |
| Experimental Models: Organisms/Strains | ||
| NSG (NOD.Cg-PrkdcScidIl2rgtm1Wjl/Sz) | Jackson laboratories | Cat#005557; RRID: IMSR_JAX:005557 |
| Oligonucleotides | ||
| GATA2 NheI F (pJW321–3xFLAG) – AATATCGCTAGCatg gag gtg gcg ccc gag cag ccg | This paper | N/A |
| GATA2 PacI R (pJW321–3xFLAG) – GGTATCTTAATTAA tcaCTAGCCCATGGCGGTCAC | This paper | N/A |
| GFI1B HpaI F (pLV-HA) – AATATCGTTAACATGCCACG CTCCTTCCTG | This paper | N/A |
| GFI1B ClaI R (pLV-HA) – GGTATCATCGATTCACTTGA GATTGTGCTGGCT | This paper | N/A |
| GFI1B EcoRI F (pFUW) – AATATCGAATTCATGCCAC GCTCCTTCCTG | This paper | N/A |
| GFI1B EcoRI R (pFUW) – GGTATCGAATTCTCACTTG AGATTGTGCTGGCT | This paper | N/A |
| FOS EcoRI F (pFUW) – AATATCGAATTCATGATGTTC TCGGGCTTCAACGCAG | This paper | N/A |
| FOS EcoRI R (pFUW) – GGTATCGAATTCTCACAGG GCCAGCAGCGTGGG | This paper | N/A |
| CR17AS 5’ – GGGATAATTTCAGCTGACTAAACAG | This paper | N/A |
| CR17AS 3’ – TTCCGTTTAGTTAGGTGCAGTTATC | This paper | N/A |
| Recombinant DNA | ||
| pFUW-M2rtTA | Pereira et al., 2013 | N/A |
| pFUW-tetO-GATA2 | Pereira et al., 2013 | N/A |
| pFUW-tetO-FOS | This paper | N/A |
| pFUW-tetO-GFI1B | This paper | N/A |
| pFUW-tetO-ETV6 | This paper | N/A |
| pLV-tetO-HA-GFI1B | This paper | N/A |
| pFUW-tetO-3xFLAG-GATA2 | This paper | N/A |
| pJW321–3xFlag-NANOG | Given by Wang lab | N/A |
| pLV-TRE-HA-GFP | Given by Lemischka lab | N/A |
| Software and Algorithms | ||
| GPSforGenes | This paper | N/A |
| FASTX tool kit | N/A | http://hannonlab.cshl.edu/fastx_toolkit/ |
| Homer | Heinz et al., 2010 | http://homer.ucsd.edu/homer/ |
| Bedtools | Quinlan and Hall, 2010 | http://bedtools.readthedocs.io/en/latest/ |
| Samtools | Li et al., 2009 | http://www.htslib.org/ |
| Cufflinks | Trapnell et al., 2012 | http://cole-trapnell-lab.github.io/cufflinks/ |
| Enrichr | Chen et al., 2013 | http://amp.pharm.mssm.edu/Enrichr/ |
| MACS1.4 | Zhang et al., 2008 | http://liulab.dfci.harvard.edu/MACS/00README.html |
| TopHat | Trapnell et al., 2009 | https://ccb.jhu.edu/software/tophat/index.shtml |
| FASTQC | N/A | https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ |
| ChromHMM | Ernst and Kellis, 2012 | http://compbio.mit.edu/ChromHMM/ |
| Limma Voom | R project for Statistical Computing | http://bioconductor.org/packages/release/bioc/html/limma.html |
| Galaxy | Galaxy server | https://usegalaxy.org |
| UCSC Genome Browser | N/A | https://genome.ucsc.edu |
| Gene set enrichment analysis (GSEA) | Subramanian et al., 2005 | http://software.broadinstitute.org/gsea/index.jsp |
| DESeq2 | R project for Statistical Computing | https://bioconductor.org/packages/release/bioc/html/DESeq2.html |
| Ilustrator | Adobe | http://www.adobe.com/cn/products/cs6/illustrator.html |
| Photoshop | Adobe | http://www.adobe.com/cn/products/cs6/photoshop.html |
| LSRII Diva Software | BD Biosciences | http://www.bdbiosciences.com/us/home |
| FlowJo Software | FlowJo, LLC | https://www.flowjo.com/ |
| GraphPad Prism 6.0 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |