| Antibodies |
| Mouse monoclonal anti-Flag (M2) |
Sigma |
Cat#F3165 |
| Rabbit polyclonal anti-Akt1/2/3 (H-136) |
Santa Cruz Biotechnology |
Cat#sc-8312 |
| Rabbit polyclonal anti-IKK1 (M-204) |
Santa Cruz Biotechnology |
Cat#sc-7184 |
| Rabbit polyclonal anti-Phospho-Akt (Ser473) |
Cell Signaling Technology |
Cat#9271 |
| Rabbit monoclonal anti-Phospho-IKK1/2 (Ser176/180) (16A6) |
Cell Signaling Technology |
Cat#2697 |
| Rabbit monoclonal anti-K63-linkage Specific Polyubiquitin (D7A11) |
Cell Signaling Technology |
Cat#5621 |
| Rabbit monoclonal anti-Ubiquitin, Lys48-Specific, clone Apu2 |
EMD Millipore |
Cat#05–1307 |
| Mouse monoclonal anti-IKK2 |
IMGNEX |
Cat#IMG-159 |
| Mouse monoclonal anti-Nuclear Matrix Protein p84 (5E10) |
GeneTex |
Cat#GTX70220 |
| Mouse monoclonal anti-GAPDH (GT239) |
GeneTex |
Cat#627408 |
| Rabbit anti-p100/p52 serum 1495 |
Dr, Nancy Rice, NCI-Frederick Cancer Research and Development |
NCI 1495 |
| Rabbit anti-Bcl3 serum 1348 |
Dr, Nancy Rice, NCI-Frederick Cancer Research and Development |
NCI 1348 |
| Rabbit anti-phospho-Ser33 Bcl3 serum |
BioBharati LifeScience (BBL), Kolkata, India |
Cat#BB-AB0090 |
| Rabbit anti-Erk2 serum |
BioBharati LifeScience (BBL), Kolkata, India |
Cat#BB-AB0135 |
| Rabbit anti-Ub serum |
BioBharati LifeScience (BBL), Kolkata, India |
Cat#BB-AB0035 |
| Rabbit anti-p50 serum |
BioBharati LifeScience (BBL), Kolkata, India |
Cat#BB-AB0080 |
| Ni-affinity resin |
BioBharati LifeScience (BBL), Kolkata, India |
Cat#BB-NA002E |
| Protein A/G plus beads |
BioBharati LifeScience (BBL), Kolkata, India |
Cat#BB-PAG001PD |
| Bacterial and Virus Strains |
| DH5a competent cells |
Invitrogen |
Cat#18265017 |
| BL21(DE3) competent cells - Novagen |
EMD Millipore |
Cat#69450 |
| Chemicals, Peptides, and Recombinant Proteins |
| Lipopolysaccharides from Escherichia coli O55:B5 |
Sigma |
Cat#L6529 |
| Cycloheximide |
Sigma |
Cat#C1988 |
| Wortmannin |
Sigma |
Cat#W1628 |
| InSolution™ MG-132 |
Calbiochem |
Cat#474791 |
| Akt Inhibitor VIII |
Calbiochem |
Cat#124018 |
| IKK Inhibitor XII |
Calbiochem |
Cat#401491 |
| Erk Inhibitor II |
Calbiochem |
Cat# FR180204
|
| TPA (12-O-Tetradecanoylphorbol-13-Acetate) |
Cell Signaling Technology |
Cat#4174 |
| Puromycin, Dihydrochloride |
EMD Millipore |
Cat#540222 |
| Polybrene Infection / Transfection Reagent |
EMD Millipore |
Cat# TR-1003-G |
| Lipofectamine 2000 Transfection Reagent |
Invitrogen |
Cat#11668–019 |
| Cell Proliferation Reagent WST-1 |
Roche |
Cat#05015944001 |
| Alkaline Phosphatase, Calf Intestinal (CIP) |
NEB |
Cat#M0290 |
| Human p52(1–408) |
Wang et al., 2012 |
N/A |
| Mouse p50(10435) |
This paper |
N/A |
| Human Flag-Bcl3(1–446) |
This paper |
N/A |
| Human His-Bcl3(1–446) wt and various mutants |
This paper |
N/A |
| Rat full-length Erk2 |
This paper |
N/A |
| Human GST-IκBα(1–54) |
This paper |
N/A |
| Human His-IκBδ(482–899) |
This paper |
N/A |
| Human His-full-length IKK1 |
Polley et al., 2013 |
N/A |
| Human His-IKK2(11–700) |
Polley et al., 2013 |
N/A |
| Critical Commercial Assays |
| Dual-Luciferase Reporter Assay System |
Promega |
Cat#E1910 |
| RNeasy Mini Kit |
QIAGEN |
Cat#74106 |
| Super reverse transcriptase reaction kit |
BioBharati LifeScience (BBL), Kolkata, India |
Cat#BB-E0043 |
| Experimental Models: Cell Lines |
| RAW 264.7 cells |
ATCC |
TIB-71 |
| HeLa cells |
ATCC |
CCL-2 |
| HeLa cells with Akt2 knock down |
This paper |
N/A |
| HeLa cells with IKK1 knock down |
This paper |
N/A |
| HeLa cells with IKK2 knock down |
This paper |
N/A |
| HeLa cells with Erk2 knock down |
This paper |
N/A |
| HEK 293T cells |
ATCC |
CRL-3216 |
| HEK 293T cells with TRAF6 knock down |
This paper |
N/A |
| U2OS cells |
ATCC |
HTB-96 |
| U2OS cells with Bcl3 knock down |
This paper |
N/A |
| U2OS cells with Bcl3 knock down + S33E/S446E |
This paper |
N/A |
| U2OS cells with Bcl3 knock down + S33E/S446A |
This paper |
N/A |
| U2OS cells with Bcl3 knock down + S33E/S114A/S446A |
This paper |
N/A |
| Oligonucleotides |
| sgRNA against Bcl3 Ser33 sequence: 5’- TGCGGGCCTTGTCGTCCCCC-3’ |
This paper |
N/A |
| shRNA target sequences for human Erk2: 5’-CAAAGTTCGAGTAGCTATCAA-3’ |
This paper |
N/A |
| shRNA target sequences for human IKK1: 5’-GCGTGCCATTGATCTATATAA-3’ |
This paper |
N/A |
| shRNA target sequences for human IKK2: 5’-GGGCAGTCTTTGCACATCA -3’ |
This paper |
N/A |
| shRNA target sequences for human TRAF6: 5’-CGGAATTTCCAGGAAACTATT-3’ |
This paper |
N/A |
| shRNA target sequences for human Akt2: 5’-AGTCTACCCTGGTGGTCATAA-3’ |
This paper |
N/A |
| Recombinant DNA |
| Plasmids: pEYFPC1-YFP+NTFlag_Bcl3(1–446) wt and various mutants |
This paper |
N/A |
| Plasmids: pEYFPC1_Bcl3(1–446) wt and various mutants |
This paper |
N/A |
| Plasmids: pLV-NTFlag_Bcl3(1–446) wt and various mutants |
This paper |
N/A |
| Plasmids: pET29b_ Bcl3(1–446) wt and various mutants |
This paper |
N/A |
| Plasmid: pCMV-Myc_TBLR1 |
Prof. Alain Chariot (University of Liege, Belgium) |
N/A |
| Plasmids: pCMV6-HA-Akt1 wt and kinase dead (K179M) |
Prof. Alexandra Newton (UCSD) |
N/A |
| Plasmid: pcDNA3-CYLD wt |
Prof. Guy Salvesen (SANFORD • BURNHAM • PREBYS Medical Discovery Institute) |
N/A |
| Plasmid: pET11a_p52(1–408) |
Wang et al., 2012 |
N/A |
| Plasmid: pET11a_p50(1–435) |
This paper |
N/A |
| Plasmid: Cyclin D1 luciferase reporter |
Wang et al., 2012 |
N/A |
| Plasmid: P-Selectin luciferase reporter |
Wang et al., 2012 |
N/A |
