Table S2. Parameters for multimodal multiphoton imaging. Selected excitation and detection spectral ranges for multimodal multiphoton imaging, the pulse shapes, targeted biomolecules and structures, and optical powers under the microscope objective.
Modality | Excitation wavelength (nm) | Pulse shape | Detection wavelength (nm) | Targeted biomolecule & structure | Optical power under objective (mW) |
---|---|---|---|---|---|
2PF | 910–970 | Transform-limited | 542–582 | FAD, elastin | 5.5 |
SHG | 1,140–1,200 | Transform-limited | 571–600 | Collagen | 10.0 |
3PF | 1,140–1,200 (week 1–5), 1,080–1,200 (week 6–9) | Transform-limited | 417–477 | NAD(P)H | 16.6 |
THG | 1,140–1,200 | Transform-limited | 381–399 | Microvesicles, lipid, nerve, collagen | 10.4 |
CARS | 780–880 (pump), 1,030–1,215 (stokes) | Optimally chirped | 642–705 | Lipid, protein, water | 11.1 (pump), 21.0 (Stokes) |