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. 2019 May;9(5):742–756. doi: 10.21037/qims.2019.04.16

Table S2. Parameters for multimodal multiphoton imaging. Selected excitation and detection spectral ranges for multimodal multiphoton imaging, the pulse shapes, targeted biomolecules and structures, and optical powers under the microscope objective.

Modality Excitation wavelength (nm) Pulse shape Detection wavelength (nm) Targeted biomolecule & structure Optical power under objective (mW)
2PF 910–970 Transform-limited 542–582 FAD, elastin 5.5
SHG 1,140–1,200 Transform-limited 571–600 Collagen 10.0
3PF 1,140–1,200 (week 1–5), 1,080–1,200 (week 6–9) Transform-limited 417–477 NAD(P)H 16.6
THG 1,140–1,200 Transform-limited 381–399 Microvesicles, lipid, nerve, collagen 10.4
CARS 780–880 (pump), 1,030–1,215 (stokes) Optimally chirped 642–705 Lipid, protein, water 11.1 (pump), 21.0 (Stokes)