Figure 1.

Different β-glucosides evoked different responses of TAS2R16 in lemurs. (a,b) HEK293T cells expressing TAS2R16 from each lemur with Gα16/gust44 were stimulated with increasing concentrations of (a) salicin and (b) arbutin. Changes in fluorescence (ΔF/F) upon ligand application were monitored (mean ± s.e.m.). Experiments were performed three to four times independently. (c,d) HEK293T cells expressing TAS2R16 of (c) the black lemur and (d) ruffed lemur with Gα16/gust44 were stimulated with increasing concentrations of arbutin in the presence of (c) 2.0 mM and (d) 3.5 mM salicin. Changes in fluorescence (ΔF/F) upon ligand application were monitored (mean ± s.e.m.). Experiments were performed three to four times independently.