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Position 2627.35 is related to the activation of lemur TAS2R16s. HEK293T cells expressing TAS2R16 mutants from the black lemur with Gα16/gust44 were stimulated with increasing concentrations of arbutin. Changes in fluorescence (ΔF/F) upon ligand application were monitored (mean ± s.e.m.). Experiments were performed three to five times independently. (Online version in colour.)
