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. 2019 Jun 5;286(1904):20190730. doi: 10.1098/rspb.2019.0730

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© 2019 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 4. — Neutral lipid accumulation in M1 macrophages. Quantification of oil red O-stained neutral lipids inside haematoxylin-stained murine bone marrow-derived macrophages (BMDMs) following stimulation with LPS and IFNγ. Initially, there were approximately 1.1 × 10⁵ BMDMs with insignificant quantities of accumulated lipid. The data presented are averaged from four experiments carried out with BMDMs derived from different mice on different days. (a) Representative images of BMDMs at 0, 12, 24, 36, 48 and 60 h after stimulation. Also shown are images of unstimulated BMDMs at 48 h and BMDMs 48 h after stimulation with LPS and staurosporin (STPN). (b) The total number of BMDMs (black circles) and the number of BMDMs that stain positively for oil red O (ORO) (grey diamonds) over time. (c) The average lipid content per cell (arbitrary units) over time. (d) The proportion of BMDMs with a range of lipid contents (arbitrary units) at 0 (black), 12 (purple), 24 (yellow), 36 (green), 48 (blue) and 60 (red) hours after stimulation with LPS and IFNγ. (Online version in colour.)