FIGURE 8.

IFN and chemokine gene signatures in kidney and salivary gland increased in disease, but are reversed by treatment with anti-CD40. (A) The IFN signature score, defined as the median expression level of the 4 IFN responsive genes (Ifi27, Ifi44, Ifi44l, and Rsad2), are graphed for young prediseased mice, PBS-treated mice, untreated baseline mice, and anti-CD40–treated mice (15 mg/kg) (n = 4–6). Blood, kidney, and salivary gland were analyzed and scored separately. (B) The chemokine signature score, defined as the median expression level of the 31 chemokine genes that were altered in the kidney or salivary gland in diseased mice listed in Supplemental Table I, are graphed for the same animals shown in (A). *p < 0.05 one-way ANOVA verses vehicle PBS, ⁺p < 0.05 one-way ANOVA verses anti-CD40.