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Nitrogen (A), Iron (B), Cobalt (C) and Nickel (D) concentrations in mature seeds of plants harvested at T2 (G5 stage). Plants were grown in lysimeters with 60 (60 N) or 160 kg of N ha−1 (160 N) and supplied with or without Si (12 kg ha−1: 60 N + Si and 160 N + Si). Values correspond to the mean ± SE for n = 3. The data obtained from plants treated with silicon (60 N + Si and 160 N + Si) were compared to their respective controls. * indicates a significant difference at p ≤ 0.05.
