Table 1.
Primers | Sequence |
---|---|
hsa-miR-1 RT | 5′-GAAAGAAGGCGAGGAGCAGATCGAGGAAGAAGACGGAAGAATGTGCGTCTCGCCTTCTTTCATGGGCAT-3′ |
hsa-miR-21 RT | 5′-GAAAGAAGGCGAGGAGCAGATCGAGGAAGAAGACGGAAGAATGTGCGTCTCGCCTTCTTTCTCAACATC-3′ |
RNU44 RT | 5′-GAAAGAAGGCGAGGAGCAGATCGAGGAAGAAGACGGAAGAATGTGCGTCTCGCCTTCTTTCAGTCAGTT-3′ |
hsa-miR-1 Forward | 5′-GCAACATACTTCTTTATATGCCCAT-3′ |
hsa-miR-21 Forward | 5′-GCGGTAGCTTATCAGACTGATGT-3′ |
RNU44 Forward | 5‘-CCTGGATGATGATAAGCAAATG-3′ |
Universal reverse | 5′-CGAGGAAGAAGACGGAAGAAT-3′ |
RT: reverse transcription, miRNA: microRNA.