Table 2.
Coefficient of determination, limit of detection (LOD), limit of quantification (LOQ), recovery, and precision of the target peptides.
| Peptide Code | R 2 | LOD a | LOQ a | Spike b | Recovery (%) | Precision (% CV) |
|---|---|---|---|---|---|---|
| SPA1-H | 0.9990 | 0.008 | 0.008 | 12500 | 109.9 | 1.86 |
| 1562.5 | 83.9 | 5.11 | ||||
| 1250 | 78.9 | 9.92 | ||||
| 1000 | 75.6 | 8.51 | ||||
| 125 | 62.5 | 7.67 | ||||
| SPA5-H | 0.9936 | 4 | 8 | 25000 | 75.8 | 3.44 |
| 3215 | 86.3 | 9.55 | ||||
| 2500 | 84.9 | 9.37 | ||||
| 2000 | 72.5 | 3.87 | ||||
| 250 | 97.8 | 17.2 | ||||
| SAP11-H | 0.9908 | 8 | 20 | 1250 | 39.8 | 11.7 |
| 156.25 | — c | — | ||||
| 125 | 68.7 | 36.4 | ||||
| 100 | 341.9 | 72.3 | ||||
| 12.5 | — | — |
a ng mL−1, b ng, c cannot be determined.