Figure 1.

The mean ± SEM of % peptide recovered from each stage of the next generation impactor for pro- and AAG-WMR, n = 3. Included are stages 1 to 7, the micro-orifice collector (MOC), the connector, throat, and filter stages, as well as the remaining peptide in the nebuliser. Each increasing stage number of the impactor has a smaller cut-off diameter, with MOC being the smallest. After nebulisation of 1 or 2 mL of 1 mg/mL peptide solution, each stage was washed with dH₂O and the washings analysed by HPLC to determine the drug deposition. The figures were used to calculate MMAD.