Table 1.
Multiple reaction monitoring transitions and parameters for pyruvic acid, TCA cycle intermediates, and deuterated citric acid (internal standard).
| Metabolite. | Parent ion (m/z) | Daughter ion (m/z) | Collision (eV) | Retention Time (min) |
|---|---|---|---|---|
| Pyruvic acid | 174 | 74.1 | 20 | 4.69 |
| Citric acid | 273 | 73.1 | 15 | 14.27 |
| Citric acid-d4 | 276 | 185 | 15 | 14.25 |
| Isocitric acid | 245 | 73.1 | 20 | 14.28 |
| cis-Aconitic acid | 229 | 147.1 | 15 | 13.41 |
| α-Ketoglutaric acid | 198 | 73.1 | 20 | 11.48 |
| Malic acid | 233 | 73.1 | 15 | 10.46 |
| Succinic acid | 247 | 147 | 15 | 8.15 |
| Fumaric acid | 245 | 73.1 | 20 | 8.56 |