Table 3.

Primer sequences used for the analysis of gene expression with real-time PCR.

Gene	Primers	5′ Primer Sequence 3′
Irs1	Forward	CAT GAG CCC CAA GAG TGT ATC
	Reverse	CCA ATG TCA GGA GAG CAA CTA C
Akt2	Forward	CCG AGT CCT ACA GAA TAC CAG
	Reverse	ACT CCA TCA CAA AGC ACA GG
Mtor	Forward	ATC CTT AAT CTG TTG CCC CG
	Reverse	ATG GTG TCT TGC AGG TAC TG
Rptor	Forward	TGG TGC CTG GAG TCA CAC TG
	Reverse	GCA CAT TCC AGG CGA TGG TG
Gapdh	Forward	GAC ATG CCG CCT GGA GAA AC
	Reverse	AGC CCA GGA TGC CCT TTA GT