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Time course study of enzymatic reaction. Extracted media from gastric cancer MKN-1 cells treated with 2 µg/mL F-ERK, 0.01 µg/mL PKCδ, 50 mM Adenosine triphosphate ATP, and incubated at different time intervals. (a) 3 h incubation; (b) 6 h incubation; (c) 12 h incubation; (d) 24 h incubation. Reproduced with the permission of [59].
