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(a) The effect of chitosan-collagen-based bio-mimic 3D matrices on hydroxyproline and collagen synthesis in bone cells; * p < 0.05 vs. CC. (b) Immunocytochemistry evaluation of collagen I expression of bone cells cultured for 14 days. Scale bars: 100 μm. CC: chitosan-collagen 3D matrix, CCH: chitosan-collagen-hydroxyapatite 3D matrix, CCCs: chitosan-collagen-chondroitin sulfate 3D matrix, and CCHCs: chitosan-collagen-hydroxyapatite-chondroitin sulfate 3D matrix.
