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Hematoxylin-eosin staining of bone cells cultured on 3D matrices, 100× magnification. Cell nuclei are stained dark purple and 3D matrices pink. Scale bars: 200 µm. Arrows show stained cells. BMMSC: bone marrow-derived mesenchymal stem cells; MC3T3-E1, pre-osteoblast. CC: chitosan-collagen 3D matrix, CCH: chitosan-collagen-hydroxyapatite 3D matrix, CCCs: chitosan-collagen-chondroitin sulfate 3D matrix, and CCHCs: chitosan-collagen-hydroxyapatite-chondroitin sulfate 3D matrix.
