Figure 3.

IGF1R is involved in the FSH-induced Protein kinase B (AKT) (Thr308) phosphorylation. (a) Immunoblots and (b) densitometric analysis of the protein bands of pAKT³⁰⁸, AKT and GADPH and (c) Immunoblots and (d) densitometric analysis of the protein bands of pAKT⁴⁷³, AKT and GADPH from SCs alone (control), or incubated with hpFSH alone or pre-treated with the IGF1R inhibitor NVP-AEW541 and/or PP1ß inhibitor tautomycin and then incubated with hpFSH. Data represent the mean ± SEM (* p < 0.05 vs. control and † p < 0.01 vs. FSH treatment alone) (one-way ANOVA) of three independent experiments, each performed in triplicate.