Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 15;151(6):727–737. doi: 10.1085/jgp.201812176

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Feng et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 5. — Correlation between mitoflash amplitude and ΔΨ_m depolarization. (A) Representative traces of mitoflashes with transient or sustained ΔΨ_m depolarization. Data were obtained in the presence of 5 mM glutamate/5 mM malate, 2.5 mM succinate, 2.5 mM ascorbate/0.5 mM TMPD, or 3 mM ATP. (B–E) Scatter plots for amplitude of cpYFP-reported mitoflashes and companion TMRM-reported ΔΨ_m depolarization. Black or red dots represent events with transient or sustained ΔΨ_m depolarization, respectively. For mitoflashes with transient ΔΨ_m depolarization under different conditions, linear regression yielded a positive correlation between the amplitude of cpYFP-reported mitoflashes and TMRM-reported ΔΨ_m depolarization supported by Complex I substrates (n = 224 events; B), Complex II substrate (n = 222 events; C), and Complex IV substrates (n = 303 events; D). Note the weak correlation between the amplitude of mitoflashes and ΔΨ_m depolarization in ATP-supported events (r = 0.10, P = 0.078, n = 292 events; E). Note that sustained ΔΨ_m depolarization was often associated with large, near-complete loss of membrane potential (red dots, n = 83, 149, 45, and 35 events for data in B, C, D, and E, respectively).