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. 2019 Apr 15;151(6):820–833. doi: 10.1085/jgp.201812291

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© 2019 Ilyaskin et al.

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Figure 6. — ATP-activated P2X4-mediated current response with single-channel events recorded from an outside-out patch. Representative continuous current trace recorded at a holding potential of −70 mV from an outside-out patch of a P2X4-expressing oocyte. Application of ATP (100 µM) is indicated by an open bar. The insets show the indicated fragments of the current trace on an expanded time scale. Binned current amplitude histograms are shown on the right side of the insets and were obtained from the corresponding parts of the trace to calculate single-channel current amplitude (i). The current level at which all channels are closed (C) was determined at the beginning of the trace before ATP application. Dotted lines in the insets indicate channel open levels (1–7).