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. 2019 Apr 15;151(6):798–819. doi: 10.1085/jgp.201812133

Table 3. Total-linker-length calculations for concatenated GABAAR constructs.

Subunit linking Total-linker-length calculation
Linked set C-term. Linker sequence N-term. End TM4 C-tail Linker N-term. to Anchor Anchor Sum
Dimeric constructs
 β2-23-α1 LYYVN Q3(Q2A3PA)2AQ5 QPSLQ WLYYV 1 23 17 ILDRL 41
 α1-10-β2 PTPHQ LQ9 QSVND WATYL 13 10 14 TVDRL 37
 α1-9a-β2 PTPHQ (AGS)3 QSVND WATYL 13 9 14 TVDRL 36
 α1-6a-β2 PTPHQ (AGS)2 QSVND WATYL 13 6 14 TVDRL 33
 α1-3a-β2 PTPHQ AGS QSVND WATYL 13 3 14 TVDRL 30
 α1-0a-β2 PTPHQ AGS NDPSN WATYL 13 3 11 TVDRL 27
 α1-(-1a)-β2 PTPHQ AGS DPSNM WATYL 13 3 10 TVDRL 26
 α1-(-2a)-β2 PTPHQ AGS PSNMS WATYL 13 3 9 TVDRL 25
 α1-(-3a)-β2 PTPHQ AGS SNMSL WATYL 13 3 8 TVDRL 24
Tetrameric and pentameric constructs
 α1first-10-β2second PTPHQ LQ9 QSVND WATYL 13 10 14 TVDRL 37
 α1first-(-1a)-β2second PTPHQ AGS DPSNM WATYL 13 3 10 TVDRL 26
 γ2first-15a-β2second SYLYL (AGS)5 QSVND SYLYL 1 15 14 TVDRL 30
 γ2first-13a-β2second SYLYL S(AGS)4 QSVND SYLYL 1 13 14 TVDRL 28
 γ2first-11a-β2second SYLYL GS(AGS)3 QSVND SYLYL 1 11 14 TVDRL 26
 γ2first-10a-β2second SYLYL S(AGS)3 QSVND SYLYL 1 10 14 TVDRL 25
 γ2first-9a-β2second SYLYL (AGS)3 QSVND SYLYL 1 9 14 TVDRL 24
 β2second-α1third LYYVN (AGS)5LGS(AGS)3 QPSLQ WLYYV 1 27 17 ILDRL 45
 α1third-β2fourth PTPHQ AGT(AGS)5 QSVND WATYL 13 18 14 TVDRL 45
 α1third-γ2fourth PTPHQ AGT QKSDD WATYL 13 3 29 ILNNL 45
 β2fourth-α1fifth LYYVN (AGS)4ATG(AGS)4 QPSLQ WLYYV 1 27 17 ILDRL 45
 γ2fourth-β2fifth SYLYL (AGS)2ATG(AGS)5 QSVND SYLYL 1 24 14 TVDRL 39

The five most proximal C-terminal amino acids (C-term.), the specific linker sequences introduced during concatenation (Linker sequence), and the five first amino acids from the predicted mature N terminus (N-term.) are presented for the indicated subunit pairs. Total-linker-lengths (Sum) are calculated as the sum of all existing amino acids and introduced linker sequence between two artificial fix points “End TM4” and “Anchor.” The five most proximal amino acids in TM4 (End TM4) and C-terminal tail lengths (C-tail) are obtained from UniProt (P14867 for α1 and P47870 for β2). The N-terminal lengths (N-term. to Anchor) are derived from a definition of an α-helix 1 anchor (Anchor) as described in the text. For tetrameric and pentameric constructs, numbers in superscript indicate the construct position of each subunit (e.g., β2second: β2 subunit in the second construct position).