Figure 2.

PPL-printed phospholipid arrays. (a) Representative fluorescence micrograph of a 6 × 10 spot array of a 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)/Rho-PE mixture printed on glass; (b) Histogram of the area distribution of 30 lipid arrays as seen in (a); (c) Example for arbitrary patterning with two inks. Printed inks were DOPC admixed with either 1 mol% Rho-PE (red channel) or 2 mol% CF-PE (green channel). Details on the alignment challenge and offset correction in two-color printing are shown in Figure S3; (d) Example of a multiplexed phospholipid array (same inks as in (c). The obtained two-color substrate was further analyzed by measuring the distances between spots within and among the different ink patterns. As shown in Figure S4, the measured distances are in accordance to the theoretical values, confirming the success of alignment and printing procedure. The scale bars in all panels equal 100 µm in the main image and 10 µm in the insets.