Table 1.
List of phosphorylated peptides in cytoplasmic and nuclear compartment identified by MS/MS analysis. The Table reports only the sites with the highest X Correlation value (Xcorr) in Sequest (1.5), the rank value of 1 and the best fragmentation pattern, selected manually after visual inspection of the MS/MS spectra. Three different tools for phosphorylation site prediction where applied: NetPhos 2.0 Server, Phosphosite plus and Phosida. Samples were analyzed by MS/MS before and after treatment with phosphates to unambiguously verify the phosphosites listed in the Table.
| Sample | PTMs | Peptide Sequence | Phosida | NetPhos 2.0 | Phosphosite Plus | Ref. [26] |
|---|---|---|---|---|---|---|
| Cyt | S98 | DGVADVSIEDSVISLSGDHCIIGRTLVVHEK | X | X | X | |
| S102 | DGVADVSIEDSVISLSGDHCIIGRTLVVHEK | X | X | |||
| S142 | TGNAGSRLACGVIGIAQ | X | ||||
| Nuc | S34 | PVKVWGSIKGLTE | X | X | ||
| T39 | GSIKGLTEGLHGF | |||||
| T58 | DNTAGCTSAGPHF | X | X | |||
| S59 | NTAGCTSAGPHFN | X | X | |||
| S69 | PHFNPLSRKHGGP | X | ||||
| S142 | TGNAGSRLACGVIGIAQ | X |