Table 3. SAXS structural parameters.

Structural parameters reported for the dimerization domain of SFPQ from the inline SEC peak (Fig. 5 ▸ a)

Guinier analysis
I(0)† (cm−1)	0.0590 ± 0.0002
R g (Å)	27.73 ± 0.12
P(r) analysis
I(0) (cm−1)	0.0590 ± 0.0001
R g (Å)	27.48 ± 0.08
d max (Å)	88.62
Porod volume (Å3)	91900
χ2 (total estimate from GNOM)	0.301
CorMap p-value	0.18
Molecular-mass determination: concentration-dependent
Partial specific volume‡ (cm3 g−1)	0.733
Contrast‡ (Δρ × 1010)	2.758
Extinction coefficient§	0.53
Calculated monomeric M r from sequence§ (kDa)	30.05
UV path length	0.3
Molecular mass M r [from I(0)]¶ (kDa)	60.58
Molecular-mass determination: concentration-independent
Molecular mass M r (MoW method)†† (kDa)	61.16
Molecular mass M r (V c method)†† (kDa)	56.08

^†For concentration-dependent molecular-mass determination, this should be multiplied by 2.05 owing to coflow measurement.

^‡Determined with MULCh (Whitten et al., 2008 ▸).

^§Determined with ProtParam (Gasteiger et al., 2005 ▸).

^¶Determined as described by Orthaber et al. (2000 ▸).

^††Molecular mass determined with PRIMUS (Franke et al., 2017 ▸).