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. 2019 Jun 10;75(Pt 6):455–460. doi: 10.1107/S2053230X19007167

Table 2. Crystallization.

Method Hanging-drop vapor diffusion
Plate type 24-well VDXm plate
Temperature (K) 293
Protein concentration (mg ml−1) 17.9 
Buffer composition of protein solution 20 mM Tris–HCl pH 8.0, 100 mM NaCl, 5 mM DTT
Composition of reservoir solution 1.33 M ammonium sulfate, 0.05 M sodium acetate pH 4.5, 0.2 M NaCl
Volume and ratio of drop 1 µl:1 µl
Volume of reservoir (µl) 250