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. 2019 May 10;75(Pt 6):405–411. doi: 10.1107/S2053230X19006046

Table 2. Crystallization.

Method Hanging-drop vapour diffusion
Plate type 24-well
Temperature (K) 293
Protein concentration (mg ml−1) 20
Buffer composition of protein solution 10 mM MgCl2, 100 mM sodium cacodylate pH 7.0
Composition of reservoir solution 18% PEG 4000, 10 mM MgCl2, 100 mM sodium cacodylate pH 7.0
Volume and ratio of drop 2 µl protein solution:2 µl reservoir solution
Volume of reservoir (ml) 1