Table 2. Crystallization.
Method | Hanging-drop vapour diffusion |
Plate type | 24-well |
Temperature (K) | 293 |
Protein concentration (mg ml−1) | 20 |
Buffer composition of protein solution | 10 mM MgCl2, 100 mM sodium cacodylate pH 7.0 |
Composition of reservoir solution | 18% PEG 4000, 10 mM MgCl2, 100 mM sodium cacodylate pH 7.0 |
Volume and ratio of drop | 2 µl protein solution:2 µl reservoir solution |
Volume of reservoir (ml) | 1 |