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. 2019 May 10;75(Pt 6):412–418. doi: 10.1107/S2053230X1900623X

Table 1. Macromolecule-production information.

Source organism Homo sapiens
DNA source Synthetic gene
Forward primer TTTTTTCCATGGGCGACCTCTCCTGTCCC
Reverse primer TTTTTTCTCGAGTTATCAATGGTGATGATGGTGATGAGAACCCCTTTCGCCCAGGTAG
Cloning vector pET-15b
Expression vector pET-15b
Expression host E. coli BL21 (DE3)
Complete amino-acid sequence of the construct produced MGDLSCPEGLEELLSAPPPDLGAQRRHGWNPKDCSENIEVKEGGLYFERRPVAQSTDGARGKRGYSRGLHAWEISWPLEQRGTHAVVGVATALAPLQTDHYAALLGSNSESWGWDIGRGKLYHQSKGPGAPQYPAGTQGEQLEVPERLLVVLDMEEGTLGYAIGGTYLGPAFRGLKGRTLYPAVSAVWGQCQVRIRYLGERGSHHHHHH

The underlined sequences are the N-terminal cloning artifacts and the C-terminal His6 tag.