Table 2. Crystallization.
| Method | Hanging-drop vapour diffusion |
| Plate type | 24-well plates |
| Temperature (K) | 289 |
| Protein concentration (mg ml−1) | 4 |
| Buffer composition of protein solution | 50 mM Tris–HCl pH 7.5, 150 mM NaCl, 5 mM DTT |
| Composition of reservoir solution | 0.2 M ammonium acetate, 0.1 M Bis-Tris pH 5.5, 25%(w/v) polyethylene glycol 3350 |
| Volume and ratio of drop (µl) | 1:1 |
| Volume of reservoir (ml) | 0.5 |