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. 2019 May 10;75(Pt 6):412–418. doi: 10.1107/S2053230X1900623X

Table 2. Crystallization.

Method Hanging-drop vapour diffusion
Plate type 24-well plates
Temperature (K) 289
Protein concentration (mg ml−1) 4
Buffer composition of protein solution 50 mM Tris–HCl pH 7.5, 150 mM NaCl, 5 mM DTT
Composition of reservoir solution 0.2 M ammonium acetate, 0.1 M Bis-Tris pH 5.5, 25%(w/v) polyethylene glycol 3350
Volume and ratio of drop (µl) 1:1
Volume of reservoir (ml) 0.5