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. 2019 Jun 13;2(3):e201900382. doi: 10.26508/lsa.201900382

Figure 4. BmGPI12 is localized to the PPM and associated with vesicles and tubules.

Figure 4.

(A, B) Immunoelectron microscopic analysis of B. microti LabS1–infected mouse erythrocytes. Ultrathin sections of high-pressure frozen and Durcupan resin–embedded infected erythrocytes were immunolabeled with anti-BmGPI12 polyclonal antibodies (amino acid 1–302). Scale bars: 500 nm (A, B). (C) Schematic diagram showing the steps in the UC of plasma samples collected from B. microti–infected mice. (D) Immunoblot analyses using preimmune (PI) serum, and anti-BmGPI12 or anti–TER-119 antibodies on either intact plasma (PL) collected from mice infected with B. microti LabS1 strain or on two fractions (supernatant: Us and pellet: Up) of plasma after UC at 120,000g. (E, F) Immunoelectron microscopic analysis of the plasma membrane fraction (Up) from mice infected with B. microti LabS1 using anti-BmGPI12 antibodies coupled to 10-nm gold particles. Scale bars: 200 nm (E), 100 nm (F). P, parasite; RBCC, red blood cell cytoplasm; RBCM, red blood cell membrane.