Skip to main content
. 2019 Jun 13;2(3):e201900382. doi: 10.26508/lsa.201900382

Figure S1. Distribution of the apical end protein BmRON2 in B. microti–infected cells.

Figure S1.

(A) Immunoblotting analysis using preimmune (PI) and anti-BmRON2 polyclonal antibodies on fractions of uninfected erythrocytes (UI) or erythrocytes infected with B. microti strain LabS1. Consistent with previous studies (Ord et al, 2016), BmRON2 (163 kD) undergoes proteolytic degradation (asterisks indicated degradation products). The 163-kD band is found in the P and S fractions but not in the H fraction consistent with the presence of BmRON2 on the surface of daughter parasites and release upon rupture of the infected erythrocyte. No signal was detected using preimmune sera. H, hemolysate; P, membrane fractions collected after saponin treatment of erythrocytes; S, mouse plasma.