. 2019 May 15;24(10):1875. doi: 10.3390/molecules24101875

Table 4.

The cytotoxic activity of tested lactones against AGS cell lines.

Lactone	Concentration [ $μ$ g/mL]						5% DMSO	${IC}_{50}$ [ $μ$ M]
Lactone	50	20	10	5	0.5	0.1	5% DMSO	${IC}_{50}$ [ $μ$ M]
5a	15 ± 0.01	18 ± 0.08	27 ± 0.10	43 ± 0.09	48 ± 0.05	54 ± 0.07	85 ± 0.1	0.0019
5b	8 ± 0.09	11 ± 0.07	14 ± 0.12	16 ± 0.06	20 ± 0.09	35 ± 0.06		0.0023
5c	10 ± 0.12	21 ± 0.08	34 ± 0.09	44 ± 0.10	47 ± 0.12	54 ± 0.12		0.0006
6a	8 ± 0.05	8 ± 0.09	19 ± 0.01	23 ± 0.09	28 ± 0.07	35 ± 0.06		0.0019
6b	8 ± 0.06	14 ± 0.07	21 ± 0.02	28 ± 0.03	35 ± 0.02	42 ± 0.02		0.0044
6c	6 ± 0.08	14 ± 0.07	16 ± 0.10	28 ± 0.06	37 ± 0.05	49 ± 0.10		0.0029
7a	8 ± 0.03	20 ± 0.06	21 ± 0.03	25 ± 0.10	26 ± 0.05	39 ± 0.02		0.0021
7b	10 ± 0.06	14 ± 0.03	20 ± 0.06	23 ± 0.02	25 ± 0.10	40 ± 0.10		0.0036
7c	8 ± 0.07	18 ± 0.10	25 ± 0.12	42 ± 0.02	49 ± 0.06	63 ± 0.03		0.0035
8a	9 ± 0.09	17 ± 0.07	25 ± 0.05	39 ± 0.09	47 ± 0.06	61 ± 0.10		0.0016
8b	11 ± 0.05	17 ± 0.10	28 ± 0.09	31 ± 0.02	35 ± 0.10	44 ± 0.15		0.0019
8c	10 ± 0.09	11 ± 0.03	18 ± 0.07	43 ± 0.10	58 ± 0.10	69 ± 0.15		0.0025
9b	8 ± 0.09	15 ± 0.06	26 ± 0.03	43 ± 0.12	52 ± 0.15	54 ± 0.15		0.0014
9c	15 ± 0.03	18 ± 0.06	23 ± 0.02	35 ± 0.10	43 ± 0.15	51 ± 0.10		0.0014
10b	10 ± 0.06	14 ± 0.08	16 ± 0.09	37 ± 0.03	43 ± 0.02	58 ± 0.07		0.0018
10c	8 ± 0.03	10 ± 0.07	13 ± 0.03	20 ± 0.02	23 ± 0.15	38 ± 0.06		0.0017
12a	9 ± 0.10	13 ± 0.08	18 ± 0.05	22 ± 0.09	26 ± 0.05	39 ± 0.09		0.0043
13a	8 ± 0.08	10 ± 0.10	18 ± 0.12	29 ± 0.05	39 ± 0.09	44 ± 0.12		0.0037

The cytotoxicity was assessed by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) reduction assay. The cell viability was calculated for four experiments including three repeats for each compound. Complete RPMI-1640 medium was used as a positive control (Control +) of cell viability (100% viable cells) and 0.03% H $_{2}$ O $_{2}$ as a negative control (Control −) of cell viability (100% dead inactive cells). All values were expressed as the mean percentage of viable cells ± SD. The differences between positive control and treated with tested compounds were evaluated by non-parametric Mann–Whitney U test. Statistical significance: p < 0.05. Dimethyl sulfoxide (DMSO) was used as a solvent; $I C_{50}$ , half maximal inhibitory concentration.