Table 1.
Number of significant metabolite changes (ANOVA; p < 0.05 and FDR < 0.05) due to different sample storage times at −80°C. Comparisons were made between storage times within subject age groups. Sample numbers per storage time group are: less than four years, n = 301; four to less than seven years, n = 300; seven to less than nine years, n = 425; nine to less than eleven years, n = 399; eleven to less than fourteen years, n = 460; and fourteen to less than sixteen years, n = 513.
| Significantly Changed Metabolites Long Storage vs. Shorter Storage within Subject Age Groups (Increase/Decrease). Inconsistencies in Percentage Sums are Due to Rounding. | ||||||
|---|---|---|---|---|---|---|
| Storage for 4 to <7 years vs. <4 years (subject age 80 years) | Storage for 9 to <11 years vs. 7 to <9 years (subject age 75 years) |
Storage for 14 to <16 years vs. 11 to <14 years (subject age 70 years) |
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| Metabolite Ontology Class (Number of Metabolites) | Number | Percent Change | Number | Percent Change | Number | Percent Change |
| All (231) | 5 (4/1) | 2 (2/0) | 59 (46/13) | 26 (20/6) | 27 (17/10) | 12 (7/4) |
| Amino acids (22) | 1 (1/0) | 5 (5/0) | 8 (4/4) | 36 (18/18) | 1 (1/0) | 5 (5/0) |
| Amino acids related (15) | 0 | 0 | 3 (2/1) | 20 (13/7) | 0 | 0 |
| Carbohydrates and related (17) | 0 | 0 | 2 (2/0) | 12 (12/0) | 2 (0/2) | 12 (0/12) |
| Complex lipids, fatty acids and related (101) | 0 | 0 | 27 (24/3) | 27 (24/3) | 15 (10/5) | 15 (10/5) |
| Energy metabolism and related (17) | 1 (1/0) | 6 (6/0) | 5 (3/2) | 29 (17/12) | 2 (1/1) | 12 (6/6) |
| Miscellaneous (8) | 1 (1/0) | 13 (13/0) | 2 (0/2) | 25 (0/25) | 0 | 0 |
| Nucleobases and related (5) | 0 | 0 | 0 | 0 | 1 (0/1) | 20 (0/20) |
| Vitamins, cofactors and related (6) | 1 (1/0) | 17 (17/0) | 1 (1/0) | 17 (17/0) | 0 | 0 |
| Unknowns (38) | 1 (0/1) | 3 (0/3) | 11 (10/1) | 29 (26/3) | 6 (5/1) | 16 (13/3) |