Table 1.
Primers used for real time-quantitative polymerase chain reaction (RT-qPCR) of key defense-related genes in citrus fruit.
| Target Gene | GenBank Accession | Forward Primer (5′–3′) | Reverse Primer (5′–3′) |
|---|---|---|---|
| β-Glu | AY971953 | ACCTCCGAAGAATCGCTTCCAA | TGTTTCTCATGGCGGGAACA |
| CHI | AF090336 | AATGATGAACGATGCCCTGCCA | CCACTTGATGCTGTCTCCAA |
| PAL | DQ088064.1 | GATTACGGATTCAAGGGTGC | TTGGTGACAGGATTGGCGAG |
| POD | AJ582678.1 | AGCCAGGAGACAATGAACAG | TAGTTTCATGGCCAGTTTGGGC |
| PPO | XM_006468155 | AACTGCTGCCCACCAAAATC | GGTCATAAGCCCCATCACAATA |
| LOX | XM_006483993 | TGACCAAGTCCAAGTTCTGCC | AACGTCTTTTGCGTCCACC |
| β-tubulin | AF052608 | GGTGCAAATCCCACCATGAA | TGGTGTCACTTGCTGCTGCCTGA |
β-Glu: β-1,3-glucanase, CHI: chitinase, PAL: phenylalanine ammonia-lyase, POD: peroxidase, PPO: polyphenol oxidase, and LOX: lipoxygenase.