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. 2019 May 11;11(5):229. doi: 10.3390/pharmaceutics11050229

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Serum norovirus (NoV)-specific antibody responses induced by equal doses of NoV VLPs and rotavirus (RV) VP6. Groups of mice immunized either with 1 µg of NoV GI.4 and GII.4-2006a VLPs or combined with 1 µg VP6 were individually tested for (A) GI.4- and (B) GII.4-2006a-specific serum IgG with two-fold dilutions starting at 1:200. Control (Ctrl) mice received carrier only. Pooled serum of both groups was tested for blocking of VLP binding using pig gastric mucin (PGM)–based assay. Shown are blocking titration curves of (C) GI.4 and (D) GII.4-2006a VLP binding. Horizontal dashed line represents 50% blocking.