Table 2.

Changes in the levels of non-phenolic metabolites in Lactuca sativa.

No.	Compound	Rol ABC (RA)			Rol C (RC)			Commercial (COM)
		↑/↓	% Change ± SEM	p Value	↑/↓	% Change ± SEM	p Value	↑/↓	% Change ± SEM	p Value
1	Leucine/Isoleucine	↑	168 ± 19.5	0.003 **	↑	457 ± 67.2	0.002 **	↑	794 ± 7.9	0.001 **
2	Glycine-Leucine	↑	57 ± 36.6	0.126	↑	69 ± 11.9	0.024 *	↑	289 ± 5.4	0.001 **
3	Glycine-Leu/Ileu-hexose	↑	2192 ± 659.5	0.015 *	↑	2039 ± 960	0.050 *	↑	2292 ± 38.7	0.001 **
4	Tryptophan	↓	22 ± 18.4	0.241	↑	107 ± 65.1	0.096	↑	494 ± 9.8	0.001 **
5	Tryptophan-hexose	↑	523 ± 193.4	0.031 *	↑	516 ± 277.1	0.071	↑	1514 ± 664.6	0.043 *
6	Tyrosine	↑	160 ± 32.9	0.007 **	↑	401 ± 76.0	0.004 **	↑	282 ± 2.9	0.001 **
7	Tyrosine-hexose	↑	688 ± 164.9	0.007 **	↑	1295 ± 41.9	0.001 **	↑	2897 ± 78.9	0.001 **
8	Phenylalanine	↑	168 ± 35.5	0.008 **	↑	540 ± 148.3	0.011 *	↑	2968 ± 13.5	0.001 **
9	Phenylalanine-hexose	↑	95 ± 16.4	0.012 *	↑	206 ± 1.8	0.001 **	↑	2567 ± 20.3	0.001 **
10	Malic acid	↓	100 ± 0.9	0.005 **	↓	100 ± 0.9	0.005 **	↓	51 ± 3.0	0.038 *
11	Citramalic acid	↓	38 ± 5.8	0.080	↓	29 ± 4.7	0.126	↑	59 ± 20.3	0.059
12	Pyroglutamic acid	↑	53 ± 10.3	0.047 *	↑	154 ± 22.8	0.004 **	↑	51 ± 2.2	0.042 *
13	Pyroglutamic acid-Leucine/Isoleucine	↑	152 ± 60.2	0.038 *	↑	294 ± 77.8	0.011 *	↑	87 ± 0.8	0.008 **
14	Uridine	↑	108 ± 44.0	0.047 *	↑	207 ± 20.5	0.001 **	↑	126 ± 3.2	0.002 **
15	Adenosine	↑	84 ± 58.1	0.124	↑	148 ± 27.3	0.007 **	↑	224 ± 1.9	0.001 **
16	Guanosine	↑	200 ± 67.7	0.024 *	↑	255 ± 54.4	0.006 **	↑	113 ± 0.2	0.003 **
17	Isopropylmalic acid	↑	56 ± 7.8	0.038 *	↑	43 ± 45.7	0.223	↑	537 ± 19.3	0.001 **
18	1,2,3,4-Tetrahydro-beta-carboline-3-carboxylic acid	↓	50 ± 9.5	0.071	↑	125 ± 96.4	0.139	↑	1698 ± 13.2	0.001 **
19	1,2,3,4-Tetrahydro-b-carboline-1,3-dicarboxylic acid	↓	38 ± 6.6	0.081	↑	147 ± 90.3	0.094	↑	1567 ± 27.7	0.001 **
20	Azelaic acid	↓	66 ± 4.8	0.022 *	↑	52 ± 52.6	0.208	↓	26 ± 1.4	0.155
21	Quinic acid	↓	61 ± 3.5	0.026 *	↓	46 ± 10.3	0.065	↓	72 ± 0.4	0.016 *
22	Citric Acid	↑	169 ± 26.6	0.004 **	↑	508 ± 137.3	0.011 *	↑	1137 ± 241.7	0.005 **
23	Amino oxononanoic acid	↑	1803 ± 388.3	0.005 **	↑	1141 ± 337.1	0.015 *	↑	188 ± 41.5	0.030 *
24	Pantothenic acid (vitamin B5)	↑	128 ± 10.0	0.003 **	↑	79 ± 167.3	0.332	↓	100 ± 0.9	0.005 **

Table shows the percentage change in levels of non-phenolic metabolites compared with untransformed (unT) Lactuca sativa plants. Values are % mean (± SEM) and P values indicate differences with unT plants (* p < 0.05, ** p < 0.01).