Table 3.
Primer set TuYV4-ORF1 used for loop-mediated isothermal amplification of Turnip yellows virus (TuYV).
| Primer | Type | Position on Genome a | Length (nt) | Sequence 5′-3′ |
|---|---|---|---|---|
| F3 | Forward outer | 897-914 | 18 | TGATGTCACCCTCCTCCG |
| B3 | Backward outer | 1084-1102 | 19 | AGTGTCCTCCTTCCGTGTG |
| FIP | Forward inner | 970-991 and 926-945 | 42 | TGCATTTTGCTAGGTTGGCAGCATTGGGAAGGACTGTTAGGC |
| BIP | Backward inner | 1019-1040 and 1064-1083 | 42 | ATGGCTGGGTTAGCGGTTATGCGCTCAGGACCATAACATCGG |
| LF2 | Loop forward outer | 946-964 | 19 | TGACGTTGGCCGCTTTACA |
| LB2 | Loop backward outer | 1041-1062 | 22 | CGAGATTGTAGGCTCAGAAGGT |
a Genome position according to the reference nucleotide sequence of TuYV isolate WA-1 (ERS2791624) [27].