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. 2019 May 16;24(10):1890. doi: 10.3390/molecules24101890

Figure 1.

Figure 1

Preparation of the nanobodies. SDS-PAGE analysis of purified SDP6 (A) and SH2 (B,C) by Ni-NTA affinity chromatography (A,B) and gel chromatography (C). Activities of SDP6 (D) and SH2 (E) were detected by ELISA. Lane M, protein molecular weight marker; Lane 1, Total proteins expressed by E.coli; Lane 2, precipitated proteins after the cells were disrupted and centrifuged; Lane 3, supernatant proteins after the cells were disrupted and centrifuged; Lanes 4–6 of SDP6 were SDP6 nanobody eluted by 60 mM imidazole; Lanes 4 and 5 were SH2 nanobody eluted by 100 mM imidazole; Lane 6 of SH2 was SH2 nanobody after gel chromatography.