Table 1.
Free amino acid profiles in developing seeds of BAT93. Metabolites related to S-methylcysteine are highlighted.
| Average Seed Weight (mg)/Develop-Mental Stage | 25/IV- Cotyledon | 52/V- Cotyledon | 105/V-Cotyledon | 157/VI- Maturation | 200/VI- Maturation | 348/VIII- Maturation | 160/Mature |
|---|---|---|---|---|---|---|---|
| Asp | 1.6 ± 0.1 | 0.80 ± 0.10 | 0.79 ± 0.02 | 1.2 ± 0.1 | 1.3 ± 0.1 | 1.4 ± 0.2 | 3.4 ± 0.4 |
| Glu | 4.5 ± 0.2 | 4.1 ± 0.2 | 4.0 ± 0.1 | 3.3 ± 0.1 | 2.9 ± 0.1 | 2.8 ± 0.1 | 4.4 ± 0.3 |
| hGSH | 0.06 ± 0.01 | 0.06 ±0.01 | 0.06 ± 0.01 | 0.12 ± 0.01 | 0.12 ± 0.02 | 0.11 ± 0.01 | 0.35 ± 0.06 |
| Ser | 1.3 ± 0.1 | 0.77 ± 0.09 | 0.56 ± 0.01 | 0.50 ± 0.01 | 0.39 ± 0.01 | 0.52 ± 0.12 | 0.24 ± 0.02 |
| His | 1.8 ± 0.1 | 1.3 ± 0.2 | 0.84 ± 0.02 | 0.98 ± 0.07 | 0.11 ± 0.01 | 0. 45 ± 0.29 | 2.5 ± 0.2 |
| γ-Glu-S-methylCys | 2.4 ± 0.1 | 7.0 ± 0.1 | 9.3 ± 0.2 | 10.2 ± 0.2 | 8.6 ± 0.3 | 11.4 ± 2.7 | 38.0 ± 2.0 |
| Gly | 0.33 ± 0.01 | 0.30 ± 0.01 | 0.25 ± 0.01 | 0.25 ± 0.01 | 0.24 ± 0.01 | 0.27 ± 0.03 | 0.58 ± 0.36 |
| Thr | 3.2 ± 0.1 | 3.3 ± 0.2 | 1.8 ± 0.1 | 1.6 ± 0.1 | 0.66 ± 0.02 | 1.3 ± 0.6 | 0.66 ± 0.06 |
| S-methylhGSH | 0.35 ± 0.02 | 0.28 ± 0.03 | 0.18 ± 0.01 | 0.17 ± 0.01 | 0.12 ± 0.01 | 0.13 ± 0.01 | 0.44 ± 0.04 |
| Arg | 5.2 ± 0.4 | 5.6 ± 0.4 | 2.9 ± 0.1 | 4.2 ± 3.2 | 0.41 ± 0.02 | 1.7 ± 1. 1 | 2.4 ± 0.2 |
| Ala | 2.6 ± 0.3 | 1.4 ± 0.1 | 1.3 ± 0. 1 | 0.82 ± 0.04 | 0.50 ± 0.02 | 0.44 ± 0.04 | 1.7± 0.1 |
| γ-Glu-Leu | 0.26 ± 0.05 | 0.39 ± 0.01 | 2.0 ± 0.1 | 1.3 ± 0.1 | 1.9 ± 0.1 | 1.1 ± 0.6 | 1.9 ± 1.4 |
| Tyr | 0.18 ± 0.03 | 0.19 ± 0.02 | 0.17 ± 0.01 | 0.13 ± 0.01 | 0.10 ± 0.01 | 0.08 ± 0.02 | 0.22 ± 0.02 |
| S-methylCys | 1.3 ± 0.1 | 1.3 ± 0. 1 | 0.43 ± 0.01 | 0.28 ± 0.01 | 0.13 ± 0.01 | 0.18 ± 0.05 | 0.32 ± 0.02 |
| Val | 1.8 ± 0.1 | 3.0 ± 0.3 | 0.94 ± 0.02 | 0.90 ± 0.05 | 0.41 ± 0.01 | 0.64 ± 0.21 | 0.93 ± 0.08 |
| Met | 2.9 ± 0.5 | 2.4 ± 0.1 | 0.75 ± 0.02 | 0.55 ± 0.03 | 0.24 ± 0.01 | 0.31 ± 0.07 | 0.38 ± 0.01 |
| Trp | 0.70 ± 0.09 | 0.38 ± 0.11 | 0.95 ± 0.03 | 0.66 ± 0.01 | 0.66 ± 0.01 | 0.51 ± 0.12 | 0.49 ± 0.01 |
| Phe | 0.47 ± 0.01 | 0.39 ± 0.05 | 0.20 ± 0.03 | 0.14 ± 0.01 | 0.09 ± 0.01 | 0.11 ± 0.02 | 0.37 ± 0.03 |
| Ile | 1.9 ± 0.1 | 3.7 ± 0.3 | 1.6 ± 0.1 | 0.90 ± 0.02 | 0.90 ± 0.01 | 0.77 ± 0.11 | 0.28 ± 0.04 |
| Leu | 4.0 ± 2.3 | 1.4 ± 0.5 | 2.2 ± 0.1 | 0.39 ± 0.38 | 0.36 ± 0.01 | 0.42 ± 0.06 | 0.26 ± 0.02 |
| Lys | 0.30 ± 0.02 | 0.59 ± 0.07 | 0.19 ± 0.01 | 0.27 ± 0.02 | 0.14 ± 0.07 | 0.19 ± 0.02 | 0.35 ± 0.03 |
Values presented are in nmol per mg seed weight; average ± standard deviation; n = 3. hGSH: homoglutathione; γ-Glu-S-methylCys: γ-glutamyl-S-methylcysteine; S-methylhGSH: S-methylhomoglutathione; S-methylCys: S-methylcysteine.