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. 2019 May 10;9(5):183. doi: 10.3390/biom9050183

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Three-dimensional atomic force microscopyAFM topography of ACT assemblies that pierce the lipid bilayer. (A) Detailed 3D topographic analysis of two types of ACT assemblies, arcs (left-hand side) and full rings (right-hand side), for which each constituent monomer has been numbered. Below, different parameters (external arc diameter, diameter, internal ring diameter, monomer diameter, monomer height and ring “hole” height) and their respective values are listed. (B) Measurement of the number of monomeric particles with a given diameter (in nm) in different ACT arc and ring assemblies.