Table 1.
Validation parameters of the used LC-MS/MS method.
| Validation Parameters * | Acrylamide | 13C3-acrylamide | HMF | |
|---|---|---|---|---|
| Calibration curve equation | y = 6421.6x − 2739.3 | y = 7114.5x − 2567.8 | y = 205.38x + 6249.7 | |
| R2 | 0.997 | 0.998 | 0.999 | |
| LOD (ng/g) | 0.62 | 0.58 | 18.9 | |
| LOQ (ng/g) | 1.89 | 1.75 | 57.5 | |
| Retention time (min) | 9.10 ± 0.07 | 9.10 ± 0.06 | 13.3 ± 0.08 | |
| RA (%) | Lower concentration | 91.2 | 91.9 | 90.0 |
| Higher concentration | 91.6 | 92.3 | 90.9 | |
| Average | 91.4 | 92.1 | 90.4 | |
| RE (%) | Lower concentration | 92.7 | 93.1 | 91.2 |
| Higher concentration | 93.0 | 93.9 | 94.2 | |
| Average | 92.8 | 93.5 | 92.7 | |
| SSE (%) | Lower concentration | 98.5 | 98.8 | 98.7 |
| Higher concentration | 98.5 | 98.3 | 96.5 | |
| Average | 98.5 | 98.5 | 97.6 | |
| RSD Intraday (%) | Lower concentration | 2.89 | 2.77 | 3.17 |
| Higher concentration | 2.86 | 2.91 | 3.15 | |
| Average | 2.88 | 2.84 | 3.16 | |
| RSD Interday (%) | Lower concentration | 3.12 | 3.01 | 3.36 |
| Higher concentration | 3.06 | 3.08 | 3.43 | |
| Average | 3.09 | 3.05 | 3.40 | |
R2—regression coefficient; LOD—limit of detection; LOQ—limit of quantification; RA—apparent recovery; RE—extraction efficiency; SSE—signal suppression/enhancement; RSD—relative standard deviation; * n = 6 for recovery and precison experiments at two concetration levels (lower concentration: 5 ng/g for acrylamide and 13C3-acrylamide and 100 ng/g for HMF; higher concentration: 10 ng/g for acrylamide and 13C3-acrylamide and 250 ng/g for HMF.