Table 1.
Selected respiratory states calculated for C2C12 myotubes.
| Compound | OXPHOS I | CII |
|---|---|---|
| CTRL | 26.91 ± 8.01 | 34.08 ± 11.09 |
| ATR | 7.28 ± 3.36 | 9.24 ± 6.22 |
| ATR + GGOH | 20.35 ± 5.23 | 20.15 ± 11.57 |
| ATR + Chol-PEG | 9.20 ± 5.13 | 10.61 ± 4.69 |
| SIM | 19.65 ± 3.74 | 23.46 ± 7.89 |
| SIM + GGOH | 22.81 ± 5.00 | 18.23 ± 6.05 |
| SIM + Chol-PEG | 19.79 ± 6.58 | 22.32 ± 6.83 |
Data were shown as mean ± SD, n = 5. All values of the oxygen consumption by mitochondria of C2C12 cells were corrected by ROX. Oxygen consumption was expressed as pmol O2 per second per 1 million cells. The significance of differences was estimated based on: One-way ANOVA test and Tukey’s test as post hoc; (OXPHOS I) p < 0.05 for CTRL vs. ATR + GGOH, ATR vs. SIM, ATR + GGOH vs. ATR + Chol; p < 0.005 for CTRL vs. ATR + Chol; p < 0.001 for CTRL vs. ATR; (C II) p < 0.01 for CTRL vs. ATR, CTRL vs. ATR + Chol; for more experimental details see ‘Material and Methods’. Legend: OXPHOS I (ADP-stimulated respiration supported Complex I), C II (mitochondrial respiration after Complex I inhibition, in the presence of succinate addition).