Fig. 6.

3-HP consumption under mixotrophic conditions. a C. necator H16 wild type, b CNCA13 (ΔmmsA1ΔmmsA2ΔmmsA3) mutant. The strains were cultivated in 240 mL serum bottles containing 24 mL of MM supplemented with 24 mM 3-HP and an atmosphere of hydrogen, carbon dioxide and air in a ratio of 8:1:1 (v/v/v). Blue circles, OD₆₀₀; green triangles, 3-HP. Error bars represent the standard deviation of the mean for three independent experiments