Fig. 5.

MV resistance assay of I239T/G354E variants. Bacteria expressing the I239T/G354E variants were tested for MV resistance in solid media. Five consecutive 10-fold dilutions of bacteria were prepared, and 4 µL of each dilution were plated on plates containing kanamycin, IPTG and 30 µg/mL MV. The ability of bacteria to form single colonies was visualized after overnight incubation. The height of the bars corresponds to the maximal dilution at which bacterial growth was observed. The experiments were repeated >3 times