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. 2019 Jun 17;9:8658. doi: 10.1038/s41598-019-45136-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Correlations of changes in gene expression with changes in UCP1. The change in UCP1 protein expression in SC WAT by acute cold treatment (post – pre) was calculated for the cold treated and contralateral legs using our previously published results⁶. The change in UCP1 protein is plotted versus the change in gene expression of genes identified by multiple correlations analysis. (A,B) The change of tryptase or CCL26 versus the change in UCP1 protein the cold leg are shown. The data were analyzed by linear regression analysis, and Pearson correlation coefficients (r) and P values are shown (n = 12). (C) The quantification of tryptase gene expression at baseline and after cold in SC WAT of the cold treated leg is shown. Data represent means ± SEM (n = 12). *P < 0.05 (Wilcoxon matched-pairs signed rank test).