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. 2019 Jun 17;9:8658. doi: 10.1038/s41598-019-45136-9

Figure 2.

Figure 2

Mast cell density and degranulation in SC WAT of research participants in response to acute cold treatment. (A,B) Tryptase staining of SC WAT at baseline and after cold (scale bar for C: 50 μm; scale bar for D: 100 μm). White arrows point to intact mast cells and yellow arrows point to mast cells with diffuse, punctate staining. (C) Quantification of mast cell density in lean subjects and subjects with obesity at baseline and in SC WAT from the cold and contralateral legs after 10 days of acute cold exposure. (D,E) Higher magnification images demonstrating degranulated and intact mast cells (scale bar: 20 μm). (F) Quantification of degranulated mast cells in lean subjects and subjects with obesity at baseline and in SC WAT from the cold and contralateral legs after 10 days of acute cold exposure. Data represent means ± SEM. Data were analyzed by RM MANOVA as described in research design and methods. **P < 0.01; ****P < 0.0001 (lean n = 17; obese n = 8).