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. Author manuscript; available in PMC: 2020 Jan 30.
Published in final edited form as: J Am Chem Soc. 2019 Jan 22;141(4):1430–1434. doi: 10.1021/jacs.8b11384

Figure 1.

Figure 1

The binding of U1A to a specific RNA sequence is used to paramagnetically study a chimera that contains the RNA sequence of interest. (A) X-ray structure (PDB ID: 1URN) of U1A (yellow; chain B) bound to a 21-nt RNA hairpin (red; chain Q). U1A side chains of S29 and S63 (green) indicate the positions used for paramagnetic tagging. (B) Construct of the RNA hairpin shown in panel A; the seven nucleotides that form close contacts with U1A are highlighted. (C) SLCA RNA construct. (D) Chimeric RNA generated from the SLCA (blue) and hairpin (red) sequences, with 15N isotopic enrichment of adenosines only (indicated in bold). All RNA constructs (panels B–D) indicate base pairing (dashes).