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. 2019 Jun 14;10:2641. doi: 10.1038/s41467-019-10645-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Cryo-electron microscopy (EM) of epsilon toxin (Etx) pores using the Volta phase plate. a Electron micrograph of a typical field of view of isolated Etx pores. Particles that were included in the final 3D map are highlighted. b Characteristic 2D class averages. Sharpened cryo-EM map of the pore coloured by monomer as visualized from the side (c) and extracellular (d) views. Scale bar corresponds to 50 nm