Fig. 8.

Model for cell cycle-dependent repression of the budding gene AXL2. In this model, AXL2 transcription is induced in the G1 phase of the cell cycle and repressed in S phase when Rnt1p is released from the nucleolus. Association of Rnt1p to the promoter stalls transcription and initiates RNA degradation. Exoribonucleases degrade the RNA cleaved by Rnt1p and function as a fail-safe to remove the RNA escaping cleavage by Rnt1p. In the G2/M phase AXL2 repression is maintained by a combination of reduced transcription initiation and increased RNA degradation. After mitosis, Rnt1p is re-localized to the nucleolus releasing the RNAPII for rapid transcription of AXL2